RDK PARAMEDICAL STUDENT STUDY

         Use of rabbit and sheep in microbiological laboratory .   Use of Rabbit             1. To obtain HIB (Ambo ccptor) for WR test.             2. To perform ASO-   titre (Rabbit RBC+ Serum+ Streptolysim→Incubated→Read                                    haemolysis   for Aso titre).               3. To perform coagulase test suspension of organism+ Rabbit plasma→ clumping means                     coagulase positive.                4. Pathogenicity test for H. influenza .          ...

What is AIDS,  & Diagnosis of AIDS in laboratory Aids stands for Acquired Immuno deficiency Syndrome. It is the end stage of disease                                        representing the breakdown of immune defence mechanism, leaving the patient pray to                        opportunistic infection and malignancies the illness progress and death ensues in month to year.                        Due to most system affected, patient present with various complain.         (a). Increasing dry cough, dyspnea and fever.       ...

  1.        Post-mortem of Lab animals Post mortem is scientific examination of tissues and organs of a dead animal or cadaver determine cause of death, extent of lesion and nature of illness. This is also called atopsy. Following changes should be noted during postmortem examination.               1. Hypostatic congestion              2. Rigor mortis              3. Decomposition and soft ening of tissue.              4. Condition of organs, viscera, bones and joints. Ø   Post mortem is also done to recover organs in which experimental material has been injected. Ø   Post mortem should perform in safety cabinot. Ø   Materials removed from post mortem should be autoclaved or incine...

                                                              FNAC FNAC stands for “fine needle Aspiration cytology”. It is a method of cytopathoLogical examination.           Modalities of FNAC: Ø   Direct vision FNAC for superficial masses. Ø   Computed tomography guided FNAC for deep seated masses. Such as intraabdominal organs, pelvic organs or masses in the brain substances.          Method of FNAC performance:- 20ml of plastic disposable syringe with 21 gauge. Fine needle of variable length are used for aspiration.                                     Tum...

  HIV ( Human immune deficiency virus ) is a lentivirus ( member of retrovirus family ) that causes AIDS in which progressive failure of immune system allow life threatening opportunistic infection and cancer to thrive. Infection with HIV occurs by the transfer of blood , semen , vaginal fluid , pre-ejaculate or breast milk. Within these body fluids HIV is present as both free virus particles and virus within infected immune cells the four major routes of transmission are:                                                  ① Unsafe sex ② Contaminated Nidles ③ Breast milk ④ Transmission from an infected mother to her baby of birth.                   ...

  Virus is obligate intracellular, non- filterable, ultra-microscopic parasite. Which require living animal, plant or bacterial cell for their reproduction. It has both characters, non- living ( outside the body / cell ) Living ( in the body )            It has some unique features such as:-        ① It has only one nucleic acid either DNA or RNA .        ② It does'nt contain energy generating enzyme ATP .        ③ Like inorganic matters, it can be cry stallised .        ④ It passes through eclipse phase during invasion into host cell .        ⑤ It is capable of bringing change in metabolic behavior of infected cell .        ⑥ Some virus produce inclusion bodies ( group of viruses in cytoplasm ) in infected cell help in their diagnosis . ...