Sterilization Sterilization means freeing of any object or subject from all living organism either in vegetative or spore that is known as to be sterilized. Sterilization can be classified as: (i). Physical method (ii). Chemical method v Physical method of sterilization: Under physical method the physical agents acts either by giving energy in the form of heat or radiation or by removing organism through filteration various physical agent are:- (a). Heat: Heat energy can be applied in two ways. (i). Dry heat (ii). Moist heat (i). Dry heat: It kills the micro organism by causing destructive oxidation of essential cell constituents or by...
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