RDK PARAMEDICAL STUDENT STUDY

                                    Describe lab diagnosis of diphtheria. Causative organism of diphtheria is coryn bacterium It is gram positive, non-motile, rod shaped, non-acid fast bacilli, non-sporing and pleomorphic. Average size Is 3 µ /0.3 µ. 1. Examination of throat swab or laryngcal swab for albert staining. Ø   Prepare smear from throat swab or laryngeal swab. Ø   Dry air and fix over flame. Ø   Pour albert-I for 5-7 mints. Ø   Blot anmd dry with the help of filter paper. Ø   Add Albert-II stain keep it for 3-5 mints. Ø   Wash under tap water. Ø   Diptheria bacilli shows met achromatic dot two pole of bacillius. 2. Culture from throat swab or laryngeal swab on. Loefflers serom agar and Tellurite blood agar. Ø   Loefflers serum agar shows gram positive bacilli with metachromatic granule in 6-8 hours. Ø   Tellurite blood ag...