Ziehl–Neelsen stain Ziehl-Neelsen staining is a type of acid-fast stain, first introduced by Paul Ehrlich. Ziehl–Neelsen staining is a bacteriological stain used to identify acid-fast organisms, mainly Mycobacteria ZN (ZIEHL-NEELSEN) Stain for AFB (ACID-FAST BACILLI) Procedure 1. Fix the smear of the specimen over the glass slide, either by heating or alcohol fixation. 2. Poor carbol fusion over smear and heat gently until fumes appear. Do not overheat and allow it to stand for 5 minutes, Then wash it off with water. 3. Pour 20% sulphuric acid, wait for one minute and keep on repeating this stepuntil the slide appears light pink in colour,...
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