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Showing posts with the label Procedure of Indole tes

Study of Indole tes

                              Indole tes Indol test is the biochemical test .This test detection of indoles such as LSD, see  Ehrlich's reagent . The  indole test  is a biochemical test performed on  bacterial species  to determine the ability of the organism to convert  tryptophan  into  indole . This division is performed by a chain of a number of different intracellular  enzymes , a system generally referred to as "tryptophanase.   Indole is generated by reductive  deamination  from tryptophan via the intermediate molecule indolepyruvic acid.  Tryptophanase  catalyzes the deamination reaction, during which the  amine  (-NH 2 ) group of the tryptophan molecule is removed. Final products of the reaction are indole,  pyruvic acid ,  ammonium ...