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Classify Viruses , Discribe Morphology Antigenic property , Transnoission and lab diagnosis of polio virus.

  Classify Viruses , Discribe Morphology Antigenic property , Transnoission and lab diagnosis of polio virus. Classification of virus :- on the basis of type of nucleic acids , viruses are classified into two group:- v   Ribo virus or RNA virus→contains RNA. v   DeoxyRibo virus or DNA virus →contains DNA . ü   RNA virus                  A ) Picorana virus (20-30) Eg→ a ) Entero virus        b ) Rhino virus        c ) Rhro virus        d ) Hepato virus (HAV)                B ) Orthomyxo virus ( 80-120nm )                    Eg:- a ) Influenza virus            ...

What disease produces by streptococci & Describe the diagnosis of Rheurnatic fever.

Diffrent types of disease of produced by streptococci There are two types of disease of produced by streptococci.         (a).superficial Lesions         (i) Cellulitis: Inflamation of interecellular substance causing appearance of fluid in subcutaneous                                 Tissue.         (ii) Erysipelals: It is appearance of erythematosus plague in epidermis.         (iii) Lymphangitis: Information of lymph vessels.         (iv) wound Infection          (b).Deep Lesions         (i) Folicular tonsillitis         (ii) Sore throat: Redne...

What is universal fixation

A tissue fixative that protects macromolecules (DNA,RNA and protein) and histomorphology in clinical samples is called universal fixative. Eg:- Formaline, Glutaraldehyde. Advantage and Disadvantage   It is a process of maintaining the tissue in the same conditions as removed from the body by preventing autolysis by keeping the tissue in a fixation solution.   Purpose of fixation: Ø   To prevent autolysis and to preserve the cell . Ø   To harden the tissue . Ø   To conserve the consistency of cells. Ø   To after refractive index of cells which enable and unstained components to be better visualized. Fixation is done in two stages: Ø   Primary fixation Ø   Secondary fixation Primary fixation is done in O.T where who organ or tissue is submerged in the fixative. Secondary fixation is done in Laboratory Large specimen are cut into slices of 1-2 cm thickness and fixed in adequate fixative for 24 hours. Next day it is cut into...

Effecting liver viruses & Hepatitis‘B’virus infection

                          E ffecting liver  viruses  & Hepatitis‘B’virus infection         Ans :   The following viruses affect liver.                                  I.             Yellow fever virus ( Arbo virus ).                                II.             Lyssa fever virus.                              I...

Describe of diphtheria.

                                    Describe lab diagnosis of diphtheria. Causative organism of diphtheria is coryn bacterium It is gram positive, non-motile, rod shaped, non-acid fast bacilli, non-sporing and pleomorphic. Average size Is 3 µ /0.3 µ. 1. Examination of throat swab or laryngcal swab for albert staining. Ø   Prepare smear from throat swab or laryngeal swab. Ø   Dry air and fix over flame. Ø   Pour albert-I for 5-7 mints. Ø   Blot anmd dry with the help of filter paper. Ø   Add Albert-II stain keep it for 3-5 mints. Ø   Wash under tap water. Ø   Diptheria bacilli shows met achromatic dot two pole of bacillius. 2. Culture from throat swab or laryngeal swab on. Loefflers serom agar and Tellurite blood agar. Ø   Loefflers serum agar shows gram positive bacilli with metachromatic granule in 6-8 hours. Ø   Tellurite blood ag...

Classification of virus

                                                  Classification of virus On the basis of type of nucleic acids , viruses are classified into two group:- v   Ribo virus or RNA virus→contains RNA. v   DeoxyRibo virus or DNA virus →contains DNA . ü   RNA virus                  A ) Picorana virus (20-30) Eg→ a ) Entero virus        b ) Rhino virus        c ) Rhro virus        d ) Hepato virus (HAV)                B ) Orthomyxo virus ( 80-120nm )                    Eg:- a ) Influenza virus   ...

Study of mycobacteria

 M ycobacterium Classification of bacteria :-    it is classified in to six groups:- A.       Tubercle Bacilli    Sn. Bacilli eg 1 HUMAN TYPE Mycobacvterium tuberculosis. 2 Bovin type Mycobacterium bovin 3 Myrin type Mycobacterium mycrotti 4 Avian type Mycobacterrium Avian 5 Cold Blooded type Mycobacterium mariumun   B.       Lepra Bacillus Sn. Bacillus eg 1 Human type Mycobacterium Leprae 2 Rat type Mycrobacterium Lapraemurium   C.       Jobenas Bacillus 1.        Mycobacterium paratuberculosis also known as mycobacterium johner. D.      ...