Rericulocyte Staining process

A reticulocyte stain is a technique a day of Highlight RNA in reticulocytes, A type oc Immuture RBC. This stain commonly use in Hematology department in pathology lab.

Edta sample mixed with Reticulocyte staining Solution(Methylene blue)

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After Mixed incubate at room temperature.

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Prepare  a blood smears

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Wait A slide Air-dried the smear

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Examined under microscope

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The number of reticulocyte count in the patient blood sample is countyed and expressed a percentage

 

The stain show the RNA in reticulocytes as a blue colour against the pink colour of the rest of the cell.

The pattern of the blue colour indicatye the stage of the reticulocyte

A hight reticulocyte count can indicate that immutur red blood cells are not surviving longenough, & that the bone marrow needs to increase production.

The reticulocyte count is an important role of monitoring of red blood cellssurvival & formation activity of bone marrow.