Skip to main content

Afb staining Procedure

Ziehl–Neelsen stain

Ziehl-Neelsen staining is a type of acid-fast stain, first introduced by Paul Ehrlich. Ziehl–Neelsen staining is a bacteriological stain used to identify acid-fast organisms, mainly Mycobacteria

                                       

ZN (ZIEHL-NEELSEN)  Stain for AFB (ACID-FAST BACILLI)  Procedure

1.      Fix the smear of the specimen over the glass slide, either by heating or alcohol fixation.

2.      Poor carbol fusion over smear and heat gently until fumes appear. Do not overheat and allow it to stand for 5 minutes, Then wash it off with water.

3.      Pour 20% sulphuric acid, wait for one minute and keep on repeating this stepuntil the slide appears light pink in colour, wash it off with water.

4.      Pour methylene blue, wait for two minutes, again wash with water.

5.      Allow it to air dry and examine under oil immersion lens.

 

Summary of acid-fast stain (Ziehl–Neelsen stain)

Application of

Reagent

Cell colour

Acid fast

Non-acid fast

Primary dye

Carbol fuchsin

Red

Red

Decolorizer

Acid alcohol

Red

Colorless

Counter stain

Methylene blue/malachite green

Red

Blue

 

                                                                                         

Number of afb count in 10 fields

Report / grading

None

Absent

1 to 2

Positive (+)

2 to 10

Positive (++)

10 to 100

Positive (+++)

Above (>) 100

Positive (++++)

 

                                                                             RANDHIR KUMAR

                                                           RDK PARAMEDICAL STUDENT STUDY

Labels:

Comments

Post a Comment

Popular posts from this blog

BIO MEDICAL WAST MANAGEMENT (BMW),

                                 BIO MEDICAL WAST MANAGEMENT (BMW) INTRODUCTION Biomedical or hospital waste refers to any waste generated while providing healthcare, performing research & undertaking investigations or related procedures on human beings or animals in hospitals, clinics, laboratories or similar establishments. Potential hazards : Biomedical waste is far more dangerous & offensive than domestic waste because. ·          It contains infectious or other hazardous materials that may injure, infect or otherwise harm patients, their   visitors, hospital personnel & the public at large in several ways. ·          It may contain sharps such as needle or broken glass, that can cause injury & infection, or harmful chemicals & radioactive materials. ·      ...
Post a Comment
Read more

HEMOGLOBIN

  HEMOGLOBIN ( Hb ) Hemoglobin is the most important component of red blood cells. It is composed of a protein called haem, which binds for carbon dioxide . Abnormilities of an individual’s hemoglobin value can indicate defects in the normal balance between red blood cell production and destruction. Both low and high values can indicate disease states. Hemoglobin is a protein in red lood cells that carries oxygen from the lungs to the human body’s   tissues   & organs,it also carries carbon dioxide back to the lungs. Hemoglobin is a most important   component of RBC(red blood cells) and gives them their colour. How is hemoglobin measured ·          A hemoglobin test   measures the amount of hemoglobin in your blood. ·          This test is usully part of a complet blood count(CBC) ·          The amount of hemoglobin in whole b...
Post a Comment
Read more

Short name to Full name of Antibiotic name

  Short name to Full name of  Antibiotic name DNA (deoxyribonucleic acid) cDNA (complementary DNA) RNA (ribonucleic acid) cRNA (complementary RNA) RNase (ribonuclease) DNase (deoxyribonuclease) rRNA (ribosomal RNA) mRNA (messenger RNA) tRNA (transfer RNA) AMP, ADP, ATP, dAMP, ddATP, and GTP, etc. (for the respective 5′ phosphates of adenosine and other nucleosides) (add 2′-, 3′-, or 5′- when needed for contrast) ATPase and dGTPase, etc. (adenosine triphosphatase and deoxyguanosine triphosphatase, etc.) NAD (nicotinamide adenine dinucleotide) NAD+ (nicotinamide adenine dinucleotide, oxidized) NADH (nicotinamide adenine dinucleotide, reduced) NADP (nicotinamide adenine dinucleotide phosphate) NADPH (nicotinamide adenine dinucleotide phosphate, reduced) NADP+ (nicotinamide adenine dinucleotide phosphate, oxidized) poly(A) and poly(dT), etc. (polyadenylic acid and polydeoxythymidylic acid, etc.) oligo(dT), etc. (oligodeoxythymidylic acid, etc.) ...
Post a Comment
Read more