RDK PARAMEDICAL STUDENT STUDY

  ANIMAL CARE Guiding Principles in the use of Animals:- → If is the primary duty of any investigator using an animal to show great eare & kindness in the animal. In some countries there are statutory provisions for the use of animal for experimental purpose. Each establishment draws up an animal house code of practice for handlers of experimental animals. This code of practice usally includes the following guidance. 1. Living condition of the animal should be appropriate for their species and should contribute to their heath & comfort. 2. Invastigators and other personnel should be duty trained in handling animals and conducting experiments on living animals. 3. Animals selected for a procedure should be of appropriate species to obtain valid result. 4. Procedure involving animals should   be designed and performed with due consideration for their relivance to human and animal health and for the good purpose. 5. When using the animals discomfort distr...

                                  CYTOLOGY Cytology:- Microscopic Exanination of indivisual cell in smear is known as cytology. → The purpose of cytology:- 1. Early diagnosis of cener. 2. Hormonal study of cytology of vaginal smear. 3. Two identify nature of lesion such as exujudate or tranjudate. 4. Sex determination in buccle smear. 5. Anti partum tuptare of membrane in pregnant ledies. Type of specimen for cytological examination 1. Urine:- urine sediment, bladder washing, prostatic massage. 2. Sputum:- Bronchial washing, brushing, brushing, expectorated sputum. 3. Gastric lavage often through gastri expiration. 4. Cervical descharges:- Lateral vaginal smear, vaginal pull smear, cervical smear, endocervical smear. 5. Breast secreation. 6. Body cavity fluid. 7. FNAC (Fine Needle Axpiration Cytology) Prepration of Smear:- 1. Prepration of smear with use of swab→ the...

    HISTOPATHOLOGY Microtome Microtome :- It is an instrument for cutting tissue section of uniform thickness. A knov on the machine is use to adjest thickness section. A knife is fixed is a clamp. Tissue block is clamped in to the microtome. If the microtome is rocking time. That block is fixed a chunk of wood. That is held in the microtome head. Microtome are different types: - 1.        Rotary Microtome: - The knife remains fixed in the microtome clamp. Paraffin block is moved up and down with the help of rotator. The tissue Advanced which each rotation. 2.        Rocking Microtome: - Tissue block remains fixed in the microtome knife is move to and fro to cut the section with each rocking tissue is moved up to allow the section cutting. 3.        Freezing Microtome: - The specimen is kept frozen by a gassous coolant which is help in a tank and is blown through a hose ove...