HISTOPATHOLOGY
Embedding
This is a process of block making, it is done in embedding
box. Counting two ‘L’ shape piece of matel the one arm of ‘L’ is longer then
the other two LR joint a for a rectangular box with acts as cast for the tissue
block.L is also known as ‘Leuchard mould’.
Process of block making
Two LR joint on a sheet of mettle or glass surface. A little
of molten p[araffin in pourd in the “L” square and is allow to cool down so
that “L” doesnot get disturubed during tissue embedding. The tissue from
paraffin bath is picked up with a tissue forcep and is place in the centre of
“L” square. Molten wax is known poured in the square molten slowly to the
filled the square up to surface it is left undisturbed for over one hours until
the paraffin cools down and becomes head. Limbs of “L” are removed the paraffin
is detached from the glass surface known this block is ready for section
cutting.
Before
section cutting knif of microtome should be made sharp. This is done a process
as honing and stropping.
Honing :- It means grinding of edge knife on a special
stone. The hon is a rectangular block of stone. It has two sides the coarse.
When is edge is buddy nicked Fine side is used when edge has minner
irragularatory the length of hon should be to present whole of the stone.
Should sufficient in support the knife.
Honing techanequic :- Clean the knife with xylene socked
cloth put on the hon back a damp.
It from sleeping during the process of honing. Clean the
surface of hon and lubricate it with coconet oil. Place the knife at one end of
the hon with edge facing away from you. Push the knife diagonally forward with the
cutting edge leading. While done this keep the knife flat to the hon by its own
weight under continue light processure.
The process of forward moved and backward move of knife should be
continue for 10 minute for cycle. The edge knife it is appears to be sharp and
smooth. Stropping is start it theedage is stillirregular and blunt honing is
repeted for the second time for another 10 minutes.
Stropping :- Stropping is a process of removing irregulatory
edge of flexable lether strop it helps removing dullness of edge of the knife.
The movement of the knife of the stropping should be indirection oppsite that
of honing. It should not have dust or absorbing material on its movement during
stropping should be rhythmic and study 22-30 dubble stropin sufficient.
