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HISTOPATHOLOGY

  HISTOPATHOLOGY Decalcification This is a process of removing calcium from bone or other mineralized hard tissue to make it soft enough for sectioning. Method of Decalcification          i.             Acid method        ii.             Ion exchange method      iii.             Chelation      iv.             Eletrical ionization. Acid method This is the common method done in most laboratory. Procedure Calcification hard tissue is cut in to small pieces (2-6mm in size) with a thin blade, or sharp knife. The cut pieces are fixed in buffered formalin or natural formalin for 8-10 minutes. Tissue is washed thoroughly in water to remove fi...

HISTOPATHOLOGY

         HISTOPATHOLOGY The study of tissue i.e. called histopathology. Microscopic study of tissue i.e. called histopathology. It deals with minute structure, composition and function of tissue. Histopathology means study of tissue disease, it needs preparation of microscopic slide of specimen remove through biopsy or surgical dissection. Specimen has to pass through following staps.          i.             Fixation        ii.             Decalcification (Remove the calcium)      iii.             Dehydration (Wash the tissue)      iv.             Cleaning        v.     ...

STUDY OF SEMEN

SEMEN ANALYSIS Introduction Problem with male semen a counts for 40% of all infertility another 40% released to female reproducts and harmon problem. The remaining 20% the pather semen Analysis is the cornerstone of testing for male in fertility this test provides important information about the quality and quantity of the sperm. Semen is a composite solution consisting basically of spermatozoa suspended in the seminal plasma. Semen is viscid neutral by slightly Alkaline and pale yellow colour due to its flavien content.   Collection of specime3n (Semen) The usually recommended specimen one collected following a three day period of continence. The most satisfactory – The specimen collected by masturbation in the clinical pathological Laboratory. This aloss a complete examination of the sperm particularly the liquification. The specimen should be delivered with in 30min in laboratory. (Note: - Wide mouth clean and dry bottle (50ml). Precaution The specimen sh...