HISTOPATHOLOGY Decalcification This is a process of removing calcium from bone or other mineralized hard tissue to make it soft enough for sectioning. Method of Decalcification i. Acid method ii. Ion exchange method iii. Chelation iv. Eletrical ionization. Acid method This is the common method done in most laboratory. Procedure Calcification hard tissue is cut in to small pieces (2-6mm in size) with a thin blade, or sharp knife. The cut pieces are fixed in buffered formalin or natural formalin for 8-10 minutes. Tissue is washed thoroughly in water to remove fi...
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