RDK PARAMEDICAL STUDENT STUDY

A tissue fixative that protects macromolecules (DNA,RNA and protein) and histomorphology in clinical samples is called universal fixative. Eg:- Formaline, Glutaraldehyde. Advantage and Disadvantage   It is a process of maintaining the tissue in the same conditions as removed from the body by preventing autolysis by keeping the tissue in a fixation solution.   Purpose of fixation: Ø   To prevent autolysis and to preserve the cell . Ø   To harden the tissue . Ø   To conserve the consistency of cells. Ø   To after refractive index of cells which enable and unstained components to be better visualized. Fixation is done in two stages: Ø   Primary fixation Ø   Secondary fixation Primary fixation is done in O.T where who organ or tissue is submerged in the fixative. Secondary fixation is done in Laboratory Large specimen are cut into slices of 1-2 cm thickness and fixed in adequate fixative for 24 hours. Next day it is cut into...